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目的 观察海水浸泡大鼠在不同低体温情况下血气和酸碱代谢的改变。方法 将 3组大鼠分别置于 19～ 2 1°C海水中游动 ,于肛温分别降至 35 ,30及 2 5°C时 ,快速自左心室取血检测血气和酸碱 ,最后断头处死动物 ,取肺组织进行 HE染色光镜观察。结果 随体温降低 ,动脉血氧分压 (Pa O2 )、肺泡内氧分压 (PAO2 )、动脉血氧分压与肺泡内氧分压比 (Pa O2 / PAO2 )、血液中氧分压与吸氧浓度之比(PO2 / FIO2 )、氧含量 (CO2 )及阴离子隙 (AG)逐渐上升 ,当大鼠体温降至 2 5°C时 (2 5°C组 ) ,Pa O2 测得值(2 4 .86± 3.6 4 ) k Pa、PAO2 (111.75± 6 .38) k Pa、Pa O2 / PAO2 1.4 4± 0 .2 9、PO2 / FIO2 834.90± 6 6 .4 8、CO2(199.0± 18.6 ) ml/ L、AG值 (2 4 .5 2± 5 .5 3) m mol/ L,与正常对照组比较 ,测得值上升非常显著 (P值均<0 .0 1) ;血浆酸碱度 (p H)、细胞外碱剩余 (BE- ECF)、全血碱剩余 (BEb)、血浆总二氧化碳 (TCO2 )、标准碳酸氢盐 (SB)、及实际碳酸氢盐 (AB)逐渐降低 ,当大鼠肛温降至 2 5°C时 (2 5°C组 ) p H达 7.2 1± 0 .0 5、BEb(- 9.97± 2 .5 1) mmol/ L、BE- ECF(- 12 .33± 2 .6 2 ) m mol/ L、TCO2 (14 .12± 2 .5 0 ) m mol/ L、SB值(15 .6 3± 2 .17) mm ol/ L、AB值 (14 .6 2?
Objective To observe the changes of blood gas and acid-base metabolism in seawater immersion rats under different hypothermia. Methods The rats in the three groups were respectively placed in the seawater at 19-21 ° C. When the rectal temperature dropped to 35, 30 and 25 ° C respectively, the blood and acid and base were rapidly taken from the left ventricle to find the final blood The animals were sacrificed and the lung tissue was taken for HE staining. Results With the decrease of body temperature, Pa O2, PAO2, PaO2 / PAO2, oxygen partial pressure The oxygen concentration (PO2 / FIO2), oxygen content (CO2) and anion gap (AG) increased gradually. When body temperature dropped to 25 ℃ (25 ℃ group) 4.86 ± 3.6 4 kPa, PAO2 (111.75 ± 6 .38) kPa, Pa O2 / PAO2 1.4 4 ± 0.29, PO2 / FIO2 834.90 ± 6.68.4, CO2 (199.0 ± 18.6) (P <0.01). The plasma pH (p <0.05) was significantly higher than that of the normal control group (P <0.05) (BE), total carbon dioxide (TCO2), standard bicarbonate (SB), and actual bicarbonate (AB) When the rectal temperature dropped to 25 ° C (25 ° C group), p H reached 7.2 ± 0. 05, BEb (-9.97 ± 2.51 mmol / L, BE-ECF (- 12.33 ± 2 .6 2) m mol / L, TCO2 (14.12 ± 2.500) m mol / L, SB value (15.63 ± 2.17) mm ol / L, AB value ?