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细菌与血小板的结合对感染性心内膜炎瓣膜赘生物的增大起关键作用,本文以定量测定法对其结合机理进行了探讨。作者选用Cowan 1等7株表型不同的金黄色葡萄球菌。各菌株首先以Mueller-Hinton肉汤(MHB)培养14小时,离心收获后以冷(4℃)0.05M Tris-HCI缓冲液(pH7.25)洗涤3次,然后以Hoechst33342荧光染料进行标记,再以泰洛氏液(pH7.4)洗涤和悬浮,使终浓度为5×10~9/ml。另取健康新西兰白兔抗凝血,分离血小板后以交
The combination of bacteria and platelets plays an important role in the increase of valve neoplasms in infective endocarditis. In this paper, the binding mechanism was discussed by quantitative assay. The authors selected Cowan 1 and other 7 strains of different Staphylococcus aureus. Each strain was first cultured in Mueller-Hinton broth (MHB) for 14 hours, centrifuged and then washed three times with cold (4 ° C) 0.05 M Tris-HCl buffer (pH7.25) and then labeled with Hoechst33342 fluorescent dye Washed and suspended with Tyrode’s solution (pH 7.4) to give a final concentration of 5 × 10 ~ 9 / ml. Another healthy New Zealand white rabbit anti-clotting, after the separation of platelets to pay