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以西兰花种子为试材,测定不同品种西兰花种子中萝卜硫苷和萝卜硫素含量,分析选取的种子在发芽过程中抗坏血酸、总酚、花色苷、萝卜硫苷、黑芥子酶活性和萝卜硫素含量的变化。结果表明,10种西兰花种子中萝卜硫苷和萝卜硫素含量差异显著,两者在LX5种子中含量最高,分别为134.06μmol/g DW和11.37 mg/g DW,且硫苷水解的产物中萝卜硫素的含量最高。LX5中,抗坏血酸在发芽过程中逐渐生成,发芽4 d时高达127.49mg/100 g FW,随后逐渐下降;总酚在发芽过程中逐渐下降,发芽5 d时其含量下降42.14%;花色苷含量与抗坏血酸含量变化趋势基本一致;萝卜硫苷和萝卜硫素含量在发芽过程中呈下降趋势;黑芥子酶活性在0~3 d无显著变化,随后逐渐增强。
Using broccoli seeds as test material, the contents of glucosinolate and sulforaphane in broccoli seeds of different varieties were determined. Ascorbic acid, total phenols, anthocyanins, glucoraphanin, myrosinase activity and radish sulfur Changes in the content of the elements. The results showed that there were significant differences in the contents of glucoraproside and sulforaphane among the 10 kinds of broccoli seeds. The contents of glucoraprosy and sulforaphane in the seeds of LX5 were the highest (134.06μmol / g DW and 11.37 mg / g DW, respectively) Sulforaphane content is the highest. LX5, ascorbic acid was gradually formed during germination, reaching as high as 127.49 mg / 100 g FW 4 days after germination, and then decreased gradually. Total phenols decreased gradually during germination and decreased 42.14% after 5 days of germination. Anthocyanin content and Ascorbic acid content changes basically the same; glucoraphanin and radish sulfur content decreased during germination; myrosinase activity in 0 ~ 3 d no significant change, and then gradually increased.