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１９９７年Ｏ’ Ｒｅｉｌｌｙ从小鼠血管内皮细胞瘤中分离到一种新的２０ ｋＤ具有特异抑制血管内皮细胞生长的抑制因子─—Ｅｎｄｏｓｔａｔｉｎ，它对牛毛细血管内皮细胞、牛肺主动脉内皮细胞有特异的抑制增殖作用，而对非血管内皮细胞系细胞如 ＮＩＨ３Ｔ３，Ａ１０平滑肌细胞等则无增殖抑制作用，实验证实大肠杆菌生产的复性及未复性的Ｅｎｄｏｓｔａｔｉｎ及杆状病毒生产的重组Ｅｎｄｏｓｔａｔｉｎ均有抑制鸡胚血管生成的作用，而且Ｅｎｄｏｓｔａｔｉｎ没有象肿瘤化疗药物那样引发肿瘤产生抗药性，其作用机理尚待进一步研究。
In 1997, O’Reilly isolated a novel 20 kD inhibitory factor that inhibits the growth of vascular endothelial cells from mouse vascular endothelial cell tumors, Endostatin, which is specific for bovine capillary endothelial cells and bovine pulmonary aorta endothelial cells. The inhibition of proliferation, but non-vascular endothelial cell lines such as NIH3T3, A10 smooth muscle cells, no proliferation inhibition, experiments confirmed that E. coli production of renatured and not renatured Endostatin and baculovirus production of recombinant Endostatin have Inhibition of chicken embryo angiogenesis, and Endostatin does not cause cancer resistance as tumor chemotherapy drugs, the mechanism of action yet to be further studied.