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目的探讨免疫组化检测Kaposi肉瘤(KS)组织人类疱疹病毒8型(HHV-8)的可行性及其诊断意义。方法采用免疫组化方法分别检测58例KS组织和40例化脓性肉芽肿组织中HHV-8的表达。结果①HHV-8在KS真皮瘤体中的表达阳性率为94.83%(55/58),在化脓性肉芽肿表达均阴性,在KS组织和血管瘤组织瘤体中的表达差异有统计学意义(P<0.05)。②HHV-8在KS组织表皮中表达阳性率为6.90%(4/58),血管内皮细胞中的表达阳性率为91.38%(53/58),真皮梭形细胞中表达阳性率为94.83%(55/58)。③结节期KS组织中表达HHV-8阳性的细胞所占比例较斑片期及斑块期明显增多,其差异有统计学意义(P<0.05)。结论免疫组化方法检测HHV-8在KS的诊断中具有重要意义。
Objective To investigate the feasibility and diagnostic significance of immunohistochemistry in the detection of Kaposi’s sarcoma (KS) tissue of human herpesvirus type 8 (HHV-8). Methods Immunohistochemistry was used to detect the expression of HHV-8 in 58 cases of KS and 40 cases of pyogenic granuloma. Results ① The positive rate of HVV-8 expression in KS dermomas was 94.83% (55/58), which was negative in all cases of purulent granuloma and was significantly different between KS tissues and hemangiomas P <0.05). ② The positive expression rate of HHV-8 in KS epidermis was 6.90% (4/58), the positive rate was 91.38% (53/58) in vascular endothelial cells and 94.83% (55.8%) in spindle cells / 58). ③ The proportion of HHV-8 positive cells in KS tissues at the nodule stage was significantly higher than that at the plaque stage and the plaque stage (P <0.05). Conclusion Immunohistochemical detection of HHV-8 in the diagnosis of KS is of great significance.