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目的尝试用双针共轴穿刺法进一步提高兔肺孤立VX2移植瘤成瘤率。方法将30只新西兰大白兔随机分为实验组和对照组,每组15只。实验组采用CT引导下双针共轴穿刺VX2瘤块植入法建立兔肺孤立移植瘤模型,对照组采用单针瘤块植入法,以大体标本及病理结果作为金标准。结果实验组兔肺内孤立VX2肿瘤成瘤率为80%,胸膜种植率为20%,平均生存时间为(67.0±7.0)d,对照组兔肺孤立肿瘤结节成瘤率为40%,胸膜种植率为53.3%,平均生存时间(53.3±10.6)d,组间差异有统计学意义(P<0.05)。结论 CT引导下双针共轴穿刺VX2瘤块植入法建立兔肺孤立移植瘤模型,较单针穿刺法明显降低了胸膜种植转移瘤,提高了肺孤立VX2肿瘤成瘤率,且实验兔生存期更长,更适于制作肺癌影像学研究的动物模型。
Objective To try to use double needle coaxial puncture method to further improve the rate of tumor formation in isolated VX2 xenografts in rabbits. Methods Thirty New Zealand white rabbits were randomly divided into experimental group and control group, with 15 in each group. In the experimental group, the model of isolated xenograft in rabbits was established by double-needle co-axial VX2 tumor implantation under CT guidance. The control group was treated with single needle tumor mass implantation, and the gross specimen and pathological results were used as the gold standard. Results The tumor-bearing rate of isolated VX2 tumors in the lungs of experimental rabbits was 80%, the pleural implantation rate was 20% and the average survival time was (67.0 ± 7.0) d. The tumor formation rate was 40% The planting rate was 53.3% and the mean survival time was 53.3 ± 10.6 days. The difference between the two groups was statistically significant (P <0.05). CONCLUSION: CT-guided VX2 tumor implants with CT-guided implants can establish an isolated xenograft model of rabbit lung. Compared with single-needle aspiration, the transplanted tumor of pleura is significantly reduced and the rate of tumor formation in isolated VX2 tumors is increased. The survival of experimental rabbits Longer, more suitable for the production of animal models of lung cancer imaging studies.