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干细胞在体外单个培养时,形成的克隆具有规则而致密的形态,这种独特的克隆被称为全克隆。近年来发现肿瘤内也存在具有干细胞特性的肿瘤细胞,即肿瘤干细胞,是肿瘤发生、复发和转移的根源。而研究证实,多种类型的肿瘤干细胞在体外单克隆培养亦可形成特征性的全克隆,这对分离富集肿瘤干细胞及深入研究其生物学特性具有重要意义。全克隆可望作为肿瘤干细胞研究一个有用的体外模型。
When the stem cells are cultured individually in vitro, the resulting clones have a regular and dense morphology. This unique clone is called a complete clone. In recent years, it has been found that there are also tumor cells with stem cell characteristics in tumor, that is, tumor stem cells, which are the root of tumor occurrence, recurrence and metastasis. The study confirmed that many types of cancer stem cells in vitro monoclonal culture can also form a characteristic full clone, which is important for the separation and enrichment of cancer stem cells and in-depth study of its biological characteristics. Full cloning is expected to be a useful in vitro model for tumor stem cell research.