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目的研究思茅红椿Toona ciliata var.henryi茎皮的化学成分及其抗炎活性。方法采用硅胶柱色谱、Sephadex LH-20柱色谱、反相C18柱色谱等分离纯化,利用理化性质和波谱学数据鉴定化合物结构,并对部分化合物进行抑制脂多糖(LPS)诱导RAW 264.7细胞释放NO活性的测定。结果从思茅红椿茎皮的95%乙醇提取物中分离得到15个化合物,分别鉴定为odoratone(1)、toonaciliatin F(2)、toonaciliatin G(3)、toonaciliatin J(4)、hispidone(5)、bourjotinolone A(6)、3-episapeline A(7)、piscidinol A(8)、aglaiodiol(9)、toonaciliatin M(10)、2α,19-dihydroxy-9-epi-ent-pimara-7,15-diene(11)、cleomiscosin B(12)、cleomiscosin C(13)、β-谷甾醇(14)、β-胡萝卜苷(15)。化合物4和10抑制LPS诱导RAW 264.7细胞释放NO的IC50值为(11.0±0.7)和(28.8±2.7)μmol/L。结论所有化合物均为首次从该植物中分离得到,化合物11~13为首次从该属植物中分离得到。其中化合物4和10对LPS诱导RAW 264.7细胞释放NO显示出了中等的抑制作用。
Objective To study the chemical constituents and anti-inflammatory activity of Toona ciliata var. Methods The compounds were isolated and purified by column chromatography on silica gel, Sephadex LH-20 column and reversed-phase C18 column. The compounds were identified by physico-chemical properties and spectral data. Some compounds were also inhibited by lipopolysaccharide (LPS) -induced RAW 264.7 cells to release NO Determination of activity. Results Fifteen compounds were isolated from the 95% ethanol extract of Simao red angelica bark and identified as odoratone (1), toonaciliatin F (2), toonaciliatin G (3), toonaciliatin J (4), hispidone , Bourjotinolone A (6), 3-episapeline A (7), piscidinol A (8), aglaiodiol (9), toonaciliatin M (10), 2alpha, 19- dihydroxy- 9-epi-ent-pimara- diene (11), cleomiscosin B (12), cleomiscosin C (13), β-sitosterol (14), β-daucosterol (15). Compounds 4 and 10 showed IC50 values of (11.0 ± 0.7) and (28.8 ± 2.7) μmol / L for LPS-induced NO release from RAW 264.7 cells. Conclusion All the compounds were isolated from this plant for the first time. Compounds 11 ~ 13 were isolated from this genus for the first time. Among them, compounds 4 and 10 showed a moderate inhibitory effect on LPS-induced NO release from RAW 264.7 cells.