Koch(1883)在患者粪便中分离出霍乱弧菌后,人们对霍乱的临床认识有了病原学基础.但对霍乱发病机理的了解却整整经历了70年.De等(1953年)在结扎的兔小肠中首次证实霍乱弧菌的无细胞滤液具有肠毒素活性,这种无细胞滤液即可产生典型米泔样便,从而明确了霍乱的症状并非由细菌本身或内毒素引起,而是由细菌的外毒素—肠毒素所致.霍乱或副霍乱弧菌不侵入小肠粘膜, Koch (1883) found that cholera was clinically etiologically based on the isolation of Vibrio cholerae in his patient’s faeces, but his understanding of the pathogenesis of cholera has lasted for nearly 70 years, with De et al (1953) The cell-free filtrate of Vibrio cholerae was first shown to have enterotoxin activity in the small intestine of rabbits. This cell-free filtrate produced typical rice flours, making it clear that the symptoms of cholera were not caused by the bacteria themselves or with endotoxins but by bacteria Of exotoxin - enterotoxin caused by V. Cholera or Vibrio cholerae do not invade the small intestine mucosa,