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为探讨 γ-谷氨酰半胱氨酸合成酶 (γ- GCS)及多药耐药相关蛋白 (MRPs)与膀胱癌化疗耐药的关系 ,应用逆转录多聚酶链式反应 (Rt- PCR)技术检测了 32例膀胱癌及其癌周正常膀胱粘膜上 γ- GCS亚单位 γ- GCSh、γ- GCSl以及 MRPs家族成员 MRP1 和 MRP2 的 m RNA的表达 ,并以 β- actin m RNA为内对照半定量进行相关分析。结果显示 :γ-GCSh、γ- GCSl、MRP1 和 MRP2 m RNA在癌组织的平均相对表达水平均高于其在正常粘膜的表达 ;γ- GCSh和 MRP1 在化疗复发病例的癌组织的平均表达水平明显高于在未接受过任何化疗的初发病例 ,同时显示两者在癌组织的表达具有较强的相关性 (r=0 .786 ,P<0 .0 1)。提示 :正常细胞的恶性化可上调 γ- GCS和 MRPs的表达 ;膀胱癌临床化疗耐药的形成与 γ- GCSh和 MRP1 的过表达密切相关 ,两者的相关性表达提示两者之间可能存在共同的调节机制
To investigate the relationship between γ-glutamylcysteine synthase (γ-GCS) and multidrug resistance-related proteins (MRPs) and chemoresistance in bladder cancer, reverse transcription-polymerase chain reaction The mRNA expression of MRP1 and MRP2 in γ-GCS subunit γ-GCSh, γ-GCSl and MRPs was detected in 32 cases of bladder cancer and normal peritumoral mucosa, and β-actin m RNA was used as internal control half Quantitative correlation analysis. The results showed that the average relative expression levels of γ-GCSh, γ-GCS1, MRP1 and MRP2 mRNA in cancer tissue were higher than those in normal mucosa. The average expression level of γ-GCSh and MRP1 in cancer tissue Which was significantly higher than those without any chemotherapy in the initial cases, also showed a strong correlation between the two expression in cancer (r = 0.786, P <0.01). It is suggested that the malignant of normal cells can up-regulate the expression of γ-GCS and MRPs. The formation of chemoresistance in bladder cancer is closely related to the overexpression of γ-GCSh and MRP1, and the correlation between them suggests that there may exist Common regulatory mechanism