论文部分内容阅读
目的:研究宫颈癌中细胞凋亡与放疗和放化疗临床效果的关系。方法:采用免疫组织化学法和脱氧核糖核酸转移酶介导的缺口末端标记(TUNEL)技术,检测60例宫颈癌患者治疗前后宫颈肿瘤细胞凋亡率。结果:在宫颈癌组织中,单纯放疗(radiotherapy,RT)前和放疗10 Gy后,肿瘤细胞凋亡率分别为33.33%和66.67%,有统计学差异(P<0.05);放化疗(synchronal radiochemotherapy,CRT)组治疗前和先行一次化疗再放疗10 Gy后,肿瘤细胞凋亡率分别为36.67%和93.33%,两者有统计学差异(P<0.01)。治疗前组间对比阳性率无统计学差异(P>0.05),治疗后组间对比,放化疗组凋亡阳性率明显高于单纯放疗组,差异有统计学意义(P<0.05),且细胞凋亡与近期临床疗效呈正相关(r=0.823,P<0.05)。结论:化疗对放射线诱导的宫颈癌细胞凋亡具有协同作用,细胞凋亡可作为宫颈癌放疗和放化疗疗效的预测指标,。
Objective: To study the relationship between apoptosis in cervical cancer and the clinical efficacy of radiotherapy and radiotherapy and chemotherapy. Methods: The apoptosis rate of cervical cancer cells in 60 patients with cervical cancer before and after treatment was detected by immunohistochemistry and DNA nick end labeling (TUNEL). Results: The apoptotic rates of cervical cancer before radiotherapy (10 Gy) and radiotherapy (10 Gy) were 33.33% and 66.67%, respectively, with statistical difference (P <0.05); radiotherapy and chemotherapy (synchronal radiochemotherapy (P <0.01). The apoptosis rates of tumor cells before treatment and after one-time chemotherapy and radiotherapy 10 Gy were 36.67% and 93.33%, respectively (P <0.01). There was no significant difference between the two groups (P> 0.05). After treatment, the positive rates of apoptosis in radiotherapy and chemotherapy group were significantly higher than those in radiotherapy group (P <0.05) There was a positive correlation between apoptosis and clinical efficacy (r = 0.823, P <0.05). Conclusion: Chemotherapy has a synergistic effect on apoptosis of cervical cancer cells induced by radiation. Apoptosis can be used as a predictor of radiotherapy and chemoradiotherapy in cervical cancer.