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目的:通过建立骨肉瘤裸鼠荷瘤模型,观察骨肉瘤中是否存在循环肿瘤细胞自我种植现象,并探讨其在骨肉瘤进展中的作用。方法:建立骨肉瘤裸鼠原位荷瘤模型后,处理组尾静脉注射红色荧光蛋白(RFP)标记循环肿瘤细胞,对照组注射PBS,2周后将处理组原位瘤体冰冻切片荧光显微镜下观察有无自我种植,并根据肿瘤生长指标(大小、重量、生长曲线)将处理组与对照组进行比较分析。结果:处理组瘤体冰冻切片后荧光显微镜下观察发现,原位瘤组织中有红色荧光散在分布,即存在循环肿瘤细胞自我种植现象。肿瘤生长各指标比较发现,处理组瘤体在大小、重量、生长速率上均显著高于对照组(P<0.05),具有统计学意义。结论:骨肉瘤中存在循环肿瘤细胞自我种植现象,并且能够促进原位灶的生长。
OBJECTIVE: To establish a tumor-bearing model of osteosarcoma in nude mice to observe whether there is self-cultivation of circulating tumor cells in osteosarcoma and to explore its role in the progress of osteosarcoma. Methods: After the osteosarcoma model was established in situ in nude mice, RFP-labeled circulating tumor cells were injected into the tail vein of the treated group and PBS was injected into the control group. After 2 weeks, the frozen sections of the treated tumors were observed under a fluorescence microscope Observed with or without self-cultivation, and according to tumor growth indicators (size, weight, growth curve) to compare the treatment group and the control group. Results: The frozen section of the treated group was observed under a fluorescence microscope. The red fluorescence was scattered in the in situ tumor tissue, that is, the self-planting of circulating tumor cells existed. The comparison of tumor growth indicators showed that the size, weight and growth rate of the treated group were significantly higher than that of the control group (P <0.05), which was statistically significant. CONCLUSIONS: There is a phenomenon of self-cultivation of circulating tumor cells in osteosarcoma and can promote the growth of in situ foci.