Objective:To establish the standardization parameters for complete pharmacoguoslic evaluation of stems of Tlespesia lampas(T.lampas)(Cav.) Dalz & Gibs(Malvaceae),an important planl in the Indian system of medicine.Methods:Morphological,microscopical,physico-chemical evaluations,florescence analysis of T.lampas stems were investigated and preliminary phytochemical analysis,GO-MS analysis and HPTLC fingerprinting were carried out for qualitative phytochemical evaluation of various extracts of stems of T.lampas.Results:Chemomieroscopy revealed the presence of lignin.starch grains and calcium oxalate crystals.Physicochemical evaluation used to detennine numerical slandards showed a result with total ash(9.03± 0.05)%w/w,acid insoluble ash(1.50±0.01)%w/w,water soluble ash(2.51±0.02)%w/w,sulphated ash(7.50+0.01)%w/w,ethanol soluble extractive(0.24±0.02)%w/w,water soluble extractive(0.08 ±0.01)<7,w/w,moisture content(6.03±0.05)%w/w and total crude fibre content of stem powder(47.36±0.32)%w/w.Behavior characteristics of the stem powder showed presence of steroids, starch,alkaloid,flavonoids and proteins.Preliminary phytochemical analysis revealed presence of glycosides,phenolic compounds,tannins,steroids,saponins,flavonoids,carbohydrates and proteins.GC-MS analysis showed the presence of fatty acids such as dodecanoic acid, lelraclecanoic acid,n-hexadecanoic acid,9-lelradecenal and HPTLC fingerprinting revealed the presence of β-sitosterol and quercetin in stems of T.lampas.Conclusions:The pharmacognostic standardization of T.lampas is useful towards establishing standards for quality, purity and sample identification. Objective: To establish the standardization parameters for complete pharmacoguoslic evaluation of stems of Tlespesia lampas (T. lampas) (Cav.) Dalz & Gibs (Malvaceae), an important planl in the Indian system of medicine. Methods: Morphological, microscopical, physico- chemical evaluations, florescence analysis of T. slamp stems were investigated and preliminary phytochemical analysis, GO-MS analysis and HPTLC fingerprinting were carried out for qualitative phytochemical evaluation of various extracts of stems of T. lampas. Results: Chemomieroscopy revealed the presence of lignin. starch granular and calcium oxalate crystals. Physicochemical evaluation used to detennine numerical slandals showed a result with total ash (9.03 ± 0.05)% w / w, acid insoluble ash (1.50 ± 0.01)% w / w, water soluble ash (0.08 ± 0.01) <7, w / w, moisture content (6.03)% w / w, sulphated ash (7.50 + 0.01)% w / w, ethanol soluble extractive (0.24 ± 0.02)% w / w ± 0.05)% w / w and total crude fiber content of stem powder (47.36 ± 0.32 )% w / w. Behavior characteristics of the stem powder showed presence of steroids, starch, alkaloid, flavonoids and proteins. Preliminary phytochemical analysis revealed glycosides, phenolic compounds, tannins, steroids, saponins, flavonoids, carbohydrates and proteins. GC- MS analysis showed the presence of fatty acids such as dodecanoic acid, lelraclecanoic acid, n-hexadecanoic acid, 9-leldecenal and HPTLC fingerprinting revealed the presence of β-sitosterol and quercetin in stems of T. lampas. Conflusions: The pharmacognostic standardization of T .lampas is useful to establishing standards for quality, purity and sample identification.