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1植物名称短距风兰(Neofinetia richardsianaChristenson)。2材料类别成熟未开裂蒴果中的种子。3培养条件(1)种子萌发培养基:1/2B_5+6-BA 1.0mg·L~(-1)(单位下同)+NAA 0.2;(2)原球茎增殖培养基:B_5+6-BA 1.0+NAA 1.5+0.1%AC;(3)分化成苗培养基:B_5+6-BA 1.0+NAA 0.2+0.05%AC;(4)壮苗和生根培养基:1/2MS+IBA 0.5+NAA 0.3+0.03%AC。以上培养基含4.0%蔗糖和0.7%琼脂,pH 5.6～5.8。培养温度为(25±2)℃,光照强度为36μmol·m~(-2)·s~(-1),光照时间为12 h·d~(-1)。
1 plant name short wind blue (Neofinetia richardsianaChristenson). 2 MATERIAL CATEGORY Seeds in mature, uncracked capsules. 3 Culture conditions (1) seed germination medium: 1 / 2B_5 + 6-BA 1.0mg · L -1 (unit same below) + NAA 0.2; (2) Protocorm proliferation medium: B_5 + 6-BA 1.0 + NAA 1.5 + 0.1% AC; (3) Differentiated into seedling medium: B_5 + 6-BA 1.0 + NAA 0.2 + 0.05% AC; (4) Strong seedling and rooting medium: 1/2 MS + IBA 0.5 + NAA 0.3 + 0.03% AC. The above medium contains 4.0% sucrose and 0.7% agar, pH 5.6 ~ 5.8. The incubation temperature was (25 ± 2) ℃, the light intensity was 36 μmol · m -2 · s -1 and the light time was 12 h · d -1.