论文部分内容阅读
为了解云南回族CSF1PO、TPOX、TH01、F13A01、FESFPS和vWA等6个STR基因座遗传多态性分布。应用复合扩增高分辨率的聚丙烯酰胺凝胶电泳分高及银染法显影技术,对云南回族上述6个STR基因座进行遗传多态性调查。结果CSF1PO基因座,11种基因型;TH01基因座,观察到6个等位基因,间种基因型;F13A01基因座,观察到7个等位基因,16种基因型;FESFPS基因座,观察到8个等位基因,22种基因型。vWA基因座,观察到7个等位基因,21种基因型,上述6个短串联重复序列基因座基因频率分布与Hardy-Weinberg平衡吻合良好。
In order to understand the genetic polymorphism distribution of six STR loci in Yunnan Hui including CSF1PO, TPOX, TH01, F13A01, FESFPS and vWA. The genetic polymorphism of the six STR loci in Yunnan Hui population was investigated by high resolution polyacrylamide gel electrophoresis with multiplex amplification and silver staining. Results CSF1PO locus and 11 genotypes; TH01 locus, 6 alleles and interspecies genotypes were observed; F13A01 locus, 7 alleles and 16 genotypes were observed; FESFPS locus was observed 8 alleles, 22 genotypes. vWA locus. Seven alleles and 21 genotypes were observed. The frequency distribution of the six short tandem repeat loci was in good agreement with Hardy-Weinberg equilibrium.