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目的:移植转染胰岛素样生长因子-1(IGF-1)的骨髓基质细胞(MSC)到心肌梗死大鼠模型中,观察心肌细胞的凋亡和心功能的变化。方法:采用结扎法制作心肌梗死大鼠模型,用含pBabe-puro/IGF-1的病毒液感染MSC,移植MSC进入梗死心肌边缘。将实验分为心肌梗死组(MI)、心梗单纯移植细胞组(MI+MSC)、心梗移植感染空质粒细胞组(MI+MSC-pBabe-puro)、心梗移植感染IGF-1质粒的细胞组(MI+MSC-pBabe-puro/hIGF-1)。免疫组化检测炎性反应的时间,决定细胞移植的时间点。术后4周,通过TUNEL法检测心肌的细胞凋亡,超声心动图检测心功能的变化。结果:免疫组化结果显示梗死后第7天的ED1细胞的阳性数比第2天明显降低(P<0.05);转染IGF-1的MSC移植到心梗的心肌中,和转染空质粒的MSC以及未作转染的MSC组比较,明显减少心肌的细胞凋亡(P<0.05);且射血分数、左室壁厚度、左室短轴缩短率明显提高(P<0.05)。结论:转染IGF-1的MSC移植到心梗大鼠心肌内,可能是通过减少心肌的细胞凋亡来改善心功能。
OBJECTIVE: To transfect bone marrow stromal cells (MSC) transfected with insulin-like growth factor-1 (IGF-1) into rat myocardial infarction model and observe the changes of cardiomyocyte apoptosis and cardiac function. Methods: The rat model of myocardial infarction was made by ligation. MSCs were infected with the virus solution containing pBabe-puro / IGF-1 and transplanted into the edge of infarcted myocardium. The experiment was divided into myocardial infarction group (MI), myocardial infarction group (MI + MSC), myocardial infarction group (MI + MSC-pBabe-puro), myocardial infarction group Cell group (MI + MSC-pBabe-puro / hIGF-1). Immunohistochemistry to detect the time of inflammatory response, determine the time point of cell transplantation. After 4 weeks, the apoptosis of myocardium was detected by TUNEL method, and the changes of cardiac function were detected by echocardiography. Results: Immunohistochemistry showed that the positive number of ED1 cells on the 7th day after infarction was significantly lower than that on the 2nd day (P <0.05). The MSC transfected with IGF-1 was transplanted into the myocardium of myocardial infarction and transfected with empty plasmid (P <0.05). The ejection fraction, left ventricular wall thickness, shortening of left ventricular shortening were significantly increased (P <0.05). CONCLUSION: Transplantation of IGF-1-loaded MSCs into the myocardium of myocardial infarction rats may improve cardiac function by decreasing myocardial apoptosis.