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目的 观察胰岛素样生长因子 (Insulin likegrowthfactor 1,IGF 1)对离体培养的少枝胶质细胞、髓鞘生长及肿瘤坏死因子 (Tumornecrosisfactor α ,TNF α)脱髓鞘后的髓鞘再生的影响 ,以期为临床治疗多发性硬化 (Multiplescrosis ,MS)提供实验依据。 方法 采用体外器官型组织培养Wistar大鼠小脑组织的方法及免疫细胞化学方法 ,观察髓鞘形成率及表达 2’3’ 环核苷酸 3’ 磷酸二酯酶 (2’3’ cyclicnucleotide ,3’ phos phodiesterase,CNPase)阳性的少突胶质细胞数。 结果 IGF 1组及IGF 1+TNFα组髓鞘形成率明显高于TNF α组 (P <0 .0 1,P <0 .0 5 ) ,18天时髓鞘形成率分别为 90 %及 75 %。CNPase阳性细胞数IGF 1组及IGF 1+TNF α组明显高于TNF α组 (P <0 .0 1)。结论 IGF 1能促进少突胶质细胞及髓鞘生长 ,并能促进TNF α脱髓鞘后的髓鞘再生。
Objective To investigate the effects of insulin like growth factor 1 (IGF1) on myelin regeneration after in vitro cultured myelin cells, myelin sheath growth and tumor necrosis factor (TNFα) demyelination, In order to provide experimental evidence for clinical treatment of multiple sclerosis (MS). Methods The myelin formation rate and the expression of 2’3 ’cyclic nucleotide (3’) gene were detected by culturing Wistar rat cerebellum in vitro and immunocytochemistry. phos phodiesterase, CNPase) positive oligodendrocytes. Results The myelination rate of IGF1 group and IGF1 + TNFα group was significantly higher than that of TNFα group (P <0.01, P <0.05). The myelination rate was 90% and 75% respectively at 18 days. The number of CNPase-positive cells in IGF1 group and IGF1 + TNFα group was significantly higher than that in TNFα group (P <0.01). Conclusion IGF 1 can promote oligodendrocyte and myelin growth and promote myelin regeneration after TNFα demyelination.