1 引言防治植物青枯病最经济有效的办法是选育抗病品种。目前在抗性评价研究中,最常使用的接种方法是茎部毛吸管滴注法和伤根接种法。毛吸管滴注法虽然比较简便可行,但由于它是直接把细菌注入到植株茎内,不能反映出细菌在自然条件下的侵染能力。伤根接种法虽然比较接近自然界情况,但由于工作量大,而且发病时间也长,所以在大量评价植物抗青枯病研究工作中和测定分析大量的菌系毒力时,它具有一定局限性。鉴于此 1 Introduction The most economical and effective way to control plant bacterial wilt is to breed resistant varieties. Currently in the resistance evaluation study, the most commonly used method of inoculation is stem hair drop instillation and wound root inoculation. Although the capillary pipetting method is simple and convenient, it injects the bacteria directly into the stem of the plant and does not reflect the bacterial’s ability to infiltrate under natural conditions. Although the wound root inoculation method is relatively close to the natural situation, it has some limitations because of the heavy workload and long time of onset, so there are some limitations when evaluating the bacterial wilt resistance in a large number of studies and in analyzing and analyzing a large number of bacterial strains . Therefore