囊虫病是人猪共患的寄生虫病,制备抗囊虫单克隆抗体有助于囊虫病的诊断和治疗。1983年R.J.We-sterwoudt等报导,人脐带血清(HUCS)在用于细胞克隆和细胞融合方面具有比胎牛血清(FCS)优越之处。如能用HUCS代替FCS,可提高细胞的融合率,并能促进细胞的增殖。我们在本工作中,比较了HUCS和FCS以及一次和二次PEG融合对结果的影响,为其他杂交瘤方面的工作提供经验。 Cysticercosis is a common parasitic disease in pigs, the preparation of anti-cysticercosis monoclonal antibody is helpful to the diagnosis and treatment of cysticercosis. 1983 R.J. We- sterwoudt et al. Reported that human umbilical cord serum (HUCS) is superior to fetal calf serum (FCS) for use in cell cloning and cell fusion. If HUCS can be used instead of FCS, the cell fusion rate can be increased and cell proliferation can be promoted. In this work, we compared the impact of HUCS and FCS and primary and secondary PEG fusion on the results, providing experience for other hybridoma work.