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目的研究肝细胞癌细胞凋亡、细胞增殖及其与病理组织学类型的关系。方法经手术病理证实的6种组织学类型肝细胞癌57例,用TUNEL法检测凋亡细胞,用免疫组化检测各标本Bcl-2、Bax,P53,Ki-67和PCNA表达。结果透明细胞型和梁索型Ki-67蛋白表达较假腺样型、实体型和低(未)分化型低(P<0.05)。假腺样型Bcl-2蛋白较低(未)分化型低(P<0.05);Bax蛋白表达梁索型较实体型、低(未)分化型和硬化型高(P<0.05);透明细胞型较低(未)分化型高(P<0.05)。Bcl-2/Bax比值梁索型较假腺样型,硬化型和低(未)分化型低(P<0.05);透明细胞型较低(未)分化型低(P<0.05)。随病理分级的升高Ki-67和PCNA蛋白表达升高(P<0.05)。3级Bax蛋白表达较2级显著降低(P<0.05)。细胞凋亡与Bax蛋白表达显著正相关,与Ki-67蛋白表达和Bcl-2/Bax蛋白表达比值显著负相关(P<0.05)。Ki-67蛋白表达与PCNA和P53蛋白表达显著正相关(P<0.05),与细胞凋亡和Bax蛋白表达显著负相关(P<0.05)。P53蛋白表达与PCNA和Ki-67蛋白表达显著正相关(P<0.05),与Bcl-2/Bax蛋白表达比值显著负相关(P<0.05)。Bcl-2蛋白表达与Bcl-2/Bax蛋白表达比值显著正相关(P<0.05),与PCNA蛋白表达显著负相关(P<0.05)。Bax蛋白表达与细胞凋亡显著正相关(P<0.05),与Ki-67、PCNA蛋白表达和Bcl-2/Bax蛋白
Objective To study the relationship between the apoptosis of hepatocellular carcinoma cells, cell proliferation and histopathological types. Methods Totally 57 hepatocellular carcinomas of six histological types confirmed by surgery and pathology were used to detect apoptotic cells by TUNEL. The expressions of Bcl-2, Bax, P53, Ki-67 and PCNA were detected by immunohistochemistry. Results The expression of Ki-67 in clear cell type and sling type was lower than that in pseudopodia, solid type and low (un) differentiated type (P <0.05). (P <0.05). Bax protein expression was significantly lower (non-differentiated) and sclerotic (P <0.05), while clear cell Type of low (not) differentiated high (P <0.05). The ratios of Bcl-2 / Bax were lower (P <0.05) than those of the gonad-like, sclerotic and low (un) differentiated ones (P <0.05). Ki-67 and PCNA protein expression increased with pathological grade (P <0.05). Grade 3 Bax protein expression was significantly lower than grade 2 (P <0.05). Apoptosis was positively correlated with Bax protein expression, but negatively correlated with Ki-67 protein expression and Bcl-2 / Bax protein expression (P <0.05). Ki-67 protein expression was positively correlated with PCNA and P53 protein expression (P <0.05), but negatively correlated with apoptosis and Bax protein expression (P <0.05). P53 protein expression was positively correlated with PCNA and Ki-67 protein expression (P <0.05), but negatively correlated with Bcl-2 / Bax protein expression (P <0.05). Bcl-2 protein expression was positively correlated with Bcl-2 / Bax protein expression (P <0.05), and negatively correlated with PCNA protein expression (P <0.05). There was a significant positive correlation between Bax protein expression and apoptosis (P <0.05), but not with Ki-67, PCNA protein expression and Bcl-2 / Bax protein