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目的使用rpo D和gyr A基因对气单胞菌进行种水平的鉴定,比较两者分类结果的差异,同时对本研究中187株气单胞菌进行较为准确的种水平的分类。方法对生化鉴定的187株气单胞菌进行rpo D和gyr A基因聚合酶链反应扩增并测序,将序列与种特异的参考序列一起构建系统发育树。结果 187株菌经rpo D基因鉴定为7个种,经gyr A基因鉴定为9个种,还有一个未定种。两者鉴定最多的3个种一致,即维氏气单胞菌温和生物变种、豚鼠气单胞菌和水族馆气单胞菌。gyr A基因鉴定增加的2个种是异嗜糖气单胞菌和简氏气单胞菌。rpo D和gyr A基因对气单胞菌鉴定一致的有174株(93%),但有13株菌(7%)两者鉴定不一致。结论 rpo D和gyr A基因能够对大部分气单胞菌进行准确一致的分类,对于两者鉴定不一致的菌株,应增加管家基因进行分析。
OBJECTIVE: To characterize Aeromonas species using rpo D and gyr A genes, and to compare the differences of their classification results. At the same time, we classified 187 Aeromonas species in this study more accurately. Methods A total of 187 Aeromonas hydrophila strains were amplified by PCR and sequenced. The sequence and species-specific reference sequences were used to construct phylogenetic tree. Results 187 strains were identified as 7 species by rpo D gene, 9 species were identified by gyr A gene and one species was undetermined. The three identified most of the two species are identical, that is, mild strains of Aeromonas sobria, Aeromonas caviae and Aquarium Aeromonas. Two of the additional gyr A gene identifications were Aeromonas hydrophila and Jane s. There were 174 (93%) identical rpo D and gyr A genes to Aeromonas, but 13 strains (7%) were not identified correctly. Conclusion The rpo D and gyr A genes can accurately and consistently classify most of Aeromonas spp. For those strains with different identification, the housekeeping gene should be added for analysis.