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为了阐明固氮系统中的固氮基因的特性,我们应用了不同固氮基因突变体的互补试验.以大肠杆菌(E.coliK12)Jc-5466的质体突变体PRD-nif~-转入肺炎克氏杆菌(K.pneumoniae)不同nif~-的受体菌中来构成Nif~-/Nif~-的杂合子.除了先前报道过的基因外,我们找到了一个在固氮系统中必要的新基因nifC.依据细菌噬菌体P_1的共转导和三点正反交试验的结果,nifC定位于nifH和nifJ之间.它们的排列顺序是his D,nif Q,nif B,nif A,nif L,nif F,nif M,nif V,nif S,nif U,nif N,nif E,nif K,nif D,nif H,nif C和nif J.从测定固氮基因的生化表型的试验指出,nif C可能是对固氮酶中的铁钼辅因子的合成或激活有关.nif H除编码固氮还原酶外,它的功能也与合成固氮酶有关.而nif J可能是nif K,nif D,nif H或nif F的表达所需要的.
In order to elucidate the characteristics of the nitrogen fixing genes in the nitrogen fixing system, we used the complementation test of different nitrogen-fixing gene mutants.We transformed the Klebsiella pneumoniae with PRD-nif ~ -, a protoplast mutant of E. coli K12 Jc-5466 In addition to the previously reported genes, we found a nifC that is necessary for the nitrogen fixation system.According to The nifC is located between nifH and nifJ as a result of the co-transduction of the bacteriophage P_1 and the three-point forward and reverse cross-hatching assay, and their sequence is his D, nif Q, nif B, nif A, nif L, nif F, nif M, nif V, nif S, nif U, nif N, nif E, nif K, nif D, nif H, nif C and nif J. From testing to determine the biochemical phenotype of a nitrogen-fixing gene, nif C may be a Enzymes are involved in the synthesis or activation of iron-molybdenum cofactors, and its function is also related to the synthesis of nitrogenase, in addition to the enzyme that codes for nitrogen-fixing reductase, whereas nif J may be the expression of nif K, nif D, nif H or nif F Needed