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目的了解2010-2015年湖北省H3N2亚型流感病毒流行特征、基因进化和抗原漂移情况。方法通过2010-2015年湖北省H3N2亚型流感病毒核酸检测阳性率划分流行高峰,分析各流行高峰病毒的阳性数、分离数和进化簇分布,结合抗原决定簇氨基酸突变位点和三维建模数据,分析3C.2a簇病毒抗原决定簇氨基酸突变位点变化情况。结果 2010-2015年湖北省H3N2亚型流感病毒存在8个流行高峰,流行高峰会受到其他型别流感流行的影响,3C.2a簇病毒主要在第Ⅶ流行高峰后流行,与3C.3a簇病毒在HA蛋白3处抗原决定簇上存在7处不同氨基酸突变位点,3C.2a簇病毒159Y-160T位点和3C.3a簇病毒159S-160K位点在三维模拟结构图中差异明显。结论 2010-2015年湖北省H3N2亚型流感病毒进化簇不断进化,加强对近期3C.2a簇病毒的进化学监测和抗原漂移分析,有助于提高湖北省流感病原学和流行病学监测水平和能力。
Objective To understand the epidemiology, gene evolution and antigen drift of H3N2 influenza virus in Hubei province from 2010 to 2015. Methods The prevalence of H3N2 subtype influenza virus in Hubei Province from 2010 to 2015 was divided into epidemic peak, and the positive number, the number of segregation and the distribution of evolutionary cluster were analyzed. Based on the amino acid mutation sites and three-dimensional modeling data , Analysis of 3C.2a cluster antigen epitopes amino acid mutation site changes. Results There were 8 epidemic peak of H3N2 subtype influenza virus in Hubei Province from 2010 to 2015. The epidemic peak was affected by the prevalence of other influenza types. 3C.2a cluster virus was mainly found after the peak of Ⅶ. There are seven different amino acid mutation sites in the epitope of HA protein 3, 159A-160T site of 3C.2a cluster virus and 159S-160K site of 3C.3a cluster virus have obvious differences in the three-dimensional simulation structure map. Conclusion The evolutionary cluster of H3N2 influenza virus in Hubei Province from 2010 to 2015 is evolving and the evolutionary monitoring and antigen drift analysis of 3C.2a cluster viruses are strengthened. It is helpful to improve the level of influenza etiology and epidemiology monitoring in Hubei Province and ability.