论文部分内容阅读
采用不同能量和剂量的N+离子束,对郑58、昌7-2和K12玉米自交系的干种子进行处理,结果表明,发芽率、发芽势和农艺性状变异都有很大差异,筛选出的N+离子束注入玉米自交系最佳诱变能量30 KV和剂量7×1016N+/cm2。同时利用10对SSR引物对玉米自交系进行扩增,共检测到39个等位基因,每对引物可检测等位基因2～5个,平均3.9个。根据SSR分子标记聚类分析,诱变能量30 kV和剂量7×1016 N+/cm2是诱变效果最佳的。3个自交系中郑58对N+离子束诱导引起的变异最敏感,N+离子束注入玉米自交系变异的生物学效应与分子标记分析结果基本一致。
The dry seeds of Zheng 58, Chang 7-2 and K12 maize inbred lines were treated with N + ion beams of different energy and dose. The results showed that the germination rate, germination potential and agronomic traits varied greatly. The best mutagenic energy of 30 KV and 7 × 10 16 N + / cm 2 were injected into maize inbred line by N + ion beam implantation. At the same time, 10 pairs of SSR primers were used to amplify maize inbred lines. A total of 39 alleles were detected, with 2 to 5 alleles per primer pair (average 3.9). According to the SSR molecular marker clustering analysis, the best mutagenic effect was obtained when the mutagenic energy was 30 kV and the dose was 7 × 1016 N + / cm2. Among the three inbred lines, Zheng 58 was the most sensitive to the variation induced by N + ion beam. The biological effect of N + ion beam implantation in maize inbred lines was basically the same as that of molecular marker analysis.