论文部分内容阅读
目的通过对照观察正常人与慢性乙型肝炎患者肝组织Caspase-3及血清谷丙转氨酶水平,探讨Caspase-3在慢性乙型肝炎发病中的意义。方法应用免疫组化方法对66例CHB患者和15例正常肝组织进行Caspase-3检测,并检测CHB患者血清谷丙转氨酶水平。结果乙型肝炎患者Caspase-3表达显著高于正常肝组织,差异有统计学意义(P<0.05)。Caspase-3表达与CHB炎症分级显著正相关(P<0.05),与血清ALT和CHB纤维化程度差异无统计学意义(P>0.05)。结论 Caspase-3表达的活性随肝炎炎症程度增高而增强,从而对慢性乙型肝炎患者肝细胞凋亡产生影响作用。
Objective To investigate the significance of Caspase-3 in the pathogenesis of chronic hepatitis B by observing the levels of Caspase-3 and serum alanine aminotransferase in normal liver tissue and chronic hepatitis B patients. Methods Immunohistochemical method was used to detect Caspase-3 in 66 CHB patients and 15 normal liver tissues, and to detect serum alanine aminotransferase levels in CHB patients. Results The expression of Caspase-3 in hepatitis B patients was significantly higher than that in normal liver tissues (P <0.05). Caspase-3 expression was significantly and positively correlated with inflammation grade of CHB (P <0.05), but not with serum ALT and CHB fibrosis degree (P> 0.05). Conclusions The activity of Caspase-3 expression is enhanced with the increase of the degree of inflammation in hepatitis B, which may affect the hepatocyte apoptosis in patients with chronic hepatitis B.