研究大鼠不同血管中α１ 肾上腺素受体（α１ａｄｒｅｎｏｃｅｐｔｏｒ，α１ＡＲ） 亚型的分布情况及其功能意义。方法：采用离体血管收缩功能实验，测定α１ＡＲ 选择性拮抗剂抑制大鼠血管ＮＥ收缩反应的功能性亲和常数（ｐＡ２） ，与分别表达α１Ａ、α１Ｂ和α１ＤＡＲ的克隆细胞上结合常数（ｐＫｉ）作相关性分析，以判断血管中的α１ＡＲ 亚型分布。结果：哌唑嗪、ＷＢ４１０１ 、５ＭＵ、ＢＭＹ７３７８ 和ＲＳ１７０５３ 分别竞争性地抑制血管对ＮＥ 的收缩效应，ｐＡ２ 值与这些拮抗剂对克隆α１Ａ、α１Ｂ、α１ＤＡＲ 的ｐＫｉ 值间的决定系数在肺动脉分别为０ ．０５、０．４５、０．７７ ；在肠系膜动脉分别为０．０２、０．４７ 、０ ．８７ ；在尾动脉分别为０ ．７７ 、０ ．７７ 、０ ．４４ ；在门静脉分别为０ ．７９ 、０ ．８１、０ ．２７。结论：大鼠肺动脉、肠系膜动脉的功能性α１ＡＲ主要为α１ＤＡＲ；而尾动脉与门静脉的功能性α１ＡＲ主要是α１Ａ和α１Ｂ亚型。 To study the distribution of α1-adrenoceptor (α1AR) subtypes in different blood vessels of rats and their functional significance. Methods: The in vitro vasoconstriction function was used to determine the functional affinity constant (pA2) of α1AR selective antagonist to inhibit the NE contraction of blood vessels and the expression of α1A, α1Band α1DAR On the binding constant (pKi) for correlation analysis to determine the vascular α1  AR subtype distribution. RESULTS: Prazosin, WB4101, 5MU, BMY7378 and RS17053 respectively competitively inhibited the contractile effect of NE on blood vessels. The determination of pA2 value and the pKi values ​​of these antagonists on the clones α1A, α1B, α1DAR Coefficients were 0 in the pulmonary artery. 05, 0.45, 0.77; in mesenteric artery, 0.02, 0.47, 0 respectively. 87; respectively 0 in the caudal artery. 77, 0. 77, 0. 44; 0 in portal vein respectively. 79, 0. 81,0. 27. Conclusions: The functional α1AR of pulmonary arteries and mesenteric arteries of rats are mostly α1DAR. The functional α1AR of caudal artery and portal vein are mainly α1A and α1B subtypes.