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目的研究农药氰戊菊酯(Fen)对雄性生殖功能系统的时间毒性及机制。方法选择健康雄性SD大鼠49只,以光照起点时间定为ZT 0,将自然时间转换为授时时间(ZT)。将大鼠分为对照组和6个Fen染毒组。Fen染毒组:以mg/kg BW的灌胃剂量分别在ZT 02、ZT 06、ZT 10、ZT 14、ZT 18、ZT 22授时时间点给药,连续30d;对照组给予等量食用调和油。制作睾丸HE病理切片,测定每日精子生成量(DSP)、检测精子活率(LSR)、畸形率(ASR)及睾丸标志酶(ACP和γ-GT)活性,应用RIA法测定大鼠睾丸中的睾酮(T)和雌二醇(E2)水平。结果 (1)与对照组相比,Fen引起大鼠睾丸组织病理性改变,且在ZT 18时损伤最为严重;Fen降低了雄性大鼠每日精子生成量、精子活率、睾丸标志酶活性和睾酮含量,增加了雌二醇含量和精子畸形率。(2)Fen引起大鼠雄性生殖指标每日精子生成量、精子活率、睾丸磷酸酶活性的变化具有昼夜节律性,其敏感时间点分别为:ZT 04、ZT 14、ZT 23。结论 Fen对雄性生殖指标具有毒性并在每日精子生成量、精子活率、睾丸磷酸酶活性中表现出一定的昼夜时间节律性。
Objective To study the time toxicity and mechanism of fenvalerate on male reproductive system. Methods Forty - nine healthy male Sprague - Dawley rats were selected and their starting time was set as ZT 0, which converted the natural time into the time of delivery (ZT). Rats were divided into control group and Fen Fen poisonous group. Fen exposure group: the dose of mg / kg BW were administered at ZT 02, ZT 06, ZT 10, ZT 14, ZT 18 and ZT 22 respectively for 30 days; the control group was given the same amount of cooking oil . The testes HE pathological sections were made and the daily sperm production (DSP), sperm motility (LSR), abnormality rate (ASR) and testis enzyme (ACP and γ-GT) Of testosterone (T) and estradiol (E2) levels. Results (1) Compared with the control group, Fen induced pathological changes of testicular tissue in rats and had the most severe damage in ZT 18. Fen reduced daily sperm production, sperm motility and testicular enzyme activity in male rats Testosterone levels increased estradiol levels and sperm deformity. (2) The daily sperm production, sperm motility and testicular phosphatase activity of male rats with Fen induced circadian rhythms. The sensitive time points were ZT 04, ZT 14 and ZT 23 respectively. Conclusion Fen was toxic to male reproductive indicators and showed a certain circadian rhythmicity in daily sperm production, sperm motility and testicular phosphatase activity.