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目的研究车前草治疗泌尿系结石的可能机理。方法水负荷法筛选尿量敏感大鼠40只,随机分为空白对照组、阳性对照组和黄酮低、中、高剂量组,每组8只。各实验组大鼠禁食不禁水15 h后,给予水负荷生理盐水30 ml/kg,30 min后阳性对照组给予氢氯噻嗪片11.6 mg/kg灌胃,黄酮低、中、高剂量组分别给予车前草总黄酮120、240、480 mg/kg灌胃,空白对照组给予等体积纯净水灌胃。第1天给药后压迫大鼠下腹,使膀胱中余尿排尽,然后置代谢笼中收集6 h尿液,比较各组大鼠1、2、4、6 h时尿重量,并检测尿液中Na+、Cl-、K+离子的含量。连续给药7天后,以乙酰胆碱和去甲肾上腺素为工具药,比较各组大鼠膀胱及尿道平滑肌收缩反应情况。结果与空白对照组比较,黄酮低、中、高剂量组均可显著增加大鼠尿重量及尿液中Na+、Cl-的含量(P<0.05,P<0.01),对K+含量无明显影响(P>0.05)。黄酮低、中、高剂量组大鼠离体膀胱平滑肌收缩率、尿道平滑肌抑制率较正常对照组和阳性对照组升高(P<0.01)。结论车前草总黄酮利尿作用明显,又可显著增强膀胱的排泄作用,其利尿兼促排的作用可能是车前草治疗泌尿系结石的重要机理之一。
Objective To study the possible mechanism of plantain before urolithiasis. Methods Forty urine-sensitive rats were screened by water load method, and randomly divided into blank control group, positive control group and low, medium and high-dose flavonoids groups, with 8 rats in each group. After fasting for 15 h, rats in each experimental group were given water loading saline 30 ml / kg. After 30 min, the positive control group was given 11.6 mg / kg hydrochlorothiazide tablets for gavage. The mice in low, medium and high dosage groups were given vehicle The former total flavonoids 120,240,480 mg / kg gavage, blank control group given equal volume of pure water gavage. On the first day after administration, the lower abdomen of the rats was compressed, the residual urine in the bladder was drained, and then 6 h of urine was collected in the metabolic cage. The urine weights of rats in each group were compared at 1, 2, 4 and 6 h, Liquid Na +, Cl-, K + ion content. Seven days after continuous administration, acetylcholine and norepinephrine were used as the experimental drugs to compare the contractile response of the bladder and urethral smooth muscle in each group. Results Compared with the blank control group, the contents of Na + and Cl- in urine of rats in low, medium and high dosage groups of flavonoids increased significantly (P <0.05, P <0.01) and had no significant effects on the content of K + P> 0.05). The contractile rate of isolated bladder smooth muscle and the inhibitory rate of smooth muscle of urethral in rats in low, middle and high dose flavonoids group were higher than those in normal control group and positive control group (P <0.01). Conclusions The total flavonoids of Psyllium have obvious diuretic effects and can significantly enhance the urinary bladder excretion. The diuretic-promoting effect may be one of the important mechanisms in the treatment of urinary calculi.