肥大细胞的染色方法很多,如甲苯胺兰、硫堇、中性红和醛复红等,其中以甲苯胺兰染色法较常用。我们在科研中发现,用经典甲苯胺兰染色法染食管粘膜组织中的肥大细胞效果不够理想,改进后,肥大细胞在淡兰色的背景中鲜明显著。现将改进的方法介绍如下材料和方法手术切除食管癌标本40例,分别固定于酒精福尔马林液(AF液)与10% Mast cells have many staining methods, such as toluidine blue, thionine, neutral red and aldehyde complex red, of which toluidine blue staining is more commonly used. We found in the research, with the classic toluidine blue dye staining in the mucosal tissue of mast cells less than ideal, improved mast cells in the light blue background obvious. The improved methods are introduced as follows Materials and Methods Surgical resection of esophageal cancer specimens 40 cases were fixed in alcohol formalin solution (AF solution) and 10%