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目的探讨人早孕绒毛组织中趋化因子CXCL16、CXCL12及其受体CXCR6、CXCR4的基因转录和蛋白表达特征。方法收集早孕绒毛组织并分离滋养细胞,采用半定量RT-PCR方法,检测人早孕绒毛滋养细胞(含绒毛滋养细胞和绒毛外滋养细胞)和人绒毛膜细胞癌细胞系JAR中CXCL16、CXCR6、CXCL12、CXCR4的基因转录水平;采用免疫组化和免疫细胞化学分析方法,检测人早孕绒毛滋养细胞及JAR细胞中CXCL16、CXCR6、CXCL12、CXCR4的蛋白表达。结果人早孕绒毛滋养细胞CXCL16、CXCR6、CXCL12、CXCR4的基因转录水平分别为0·89±0·11、1·12±0·25、0·78±0·10、1·08±0·11;JAR细胞CXCL16、CXCR6、CXCL12、CXCR4的基因转录水平分别为0·90±0·21、1·00±0·30、0·66±0·13、0·90±0·18,两者比较,差异无统计学意义(P>0·05)。CXCL16、CXCR6、CXCL12、CXCR4蛋白在人早孕绒毛滋养细胞和绒毛外滋养细胞中的表达均呈阳性。结论人早孕绒毛滋养细胞同时表达CXCL16、CXCL12、CXCR6、CXCR4,提示母-胎界面存在复杂的趋化因子调控网络,并参与调控滋养细胞自身的生物学行为。
Objective To investigate the gene transcription and protein expression of chemokines CXCL16, CXCL12, CXCR6 and CXCR4 in human chorionic villi in early pregnancy. Methods The chorionic tissue of early pregnancy was collected and the cells were isolated. Semi-quantitative RT-PCR was used to detect CXCL16, CXCR6 and CXCL12 in JAR of human chorionic villi and villous trophoblast cells , CXCR4 gene transcription levels were detected by immunohistochemistry and immunocytochemistry. The protein expressions of CXCL16, CXCR6, CXCL12 and CXCR4 in human gestational trophoblast cells and JAR cells were detected by immunohistochemistry. Results The gene transcription levels of CXCL16, CXCR6, CXCL12 and CXCR4 in gestational trophoblast cells in early pregnancy were 0.89 ± 0.11, 1.12 ± 0.25, 0.78 ± 0.10, 1.08 ± 0.11 ; The transcriptional levels of CXCL16, CXCR6, CXCL12 and CXCR4 in JAR cells were 0.90 ± 0.21, 1.00 ± 0.30, 0.66 ± 0.13 and 0.90 ± 0.18, respectively The difference was not statistically significant (P> 0.05). The expression of CXCL16, CXCR6, CXCL12 and CXCR4 protein in human early pregnancy villous trophoblasts and extravillous trophoblast cells were all positive. Conclusions Human trophoblast cells express CXCL16, CXCL12, CXCR6 and CXCR4 at the same time, suggesting that there are complicated chemotactic regulatory networks at the maternal-fetal interface and are involved in the regulation of the biological behavior of trophoblasts.