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以香蕉交脉蚜（Ｐｅｎｔａｌｏｎｉａｎｉｇｒｏｎｃｒｖｏｓａ）人工接种表现典型束顶病症状的香蕉组培苗为材料，预预先经氯仿－正丁醇充分乳化的缓冲液抽提和澄清、差速离心、蔗糖垫部分纯化、蔗糖梯度离心，得到粒体完整的直径约１８－２０ｎｍ的球状病毒。提纯的病毒制剂具典型的核蛋白紫外吸收光谱，最高吸收峰在２５７ｎｍ左右，最低吸收峰在２４０ｎｍ左右，Ａ２６０／２８０＝１．３，提纯产量最高可达３．４ｍｇ／ｋｇ组织。用上述提纯病毒作为抗原免疫家兔制备的抗血清，经对流免疫电泳方法测定效价为１：３２。用该抗血清建立的Ａ蛋白夹心ＥＬＩＳＡ（ＤＡＳ－ＥＬＩＳＡ）及与单克隆抗体结合使用的异种抗体双夹心ＥＬＩＳＡ（ＤＡＳ－ＥＬＩＳＡ）能检测各种香蕉束顶病样品。
A banana tissue culture seedling exhibiting typical symptoms of toadstool was artificially inoculated with Pentalonian groncrvosa, and was pre-extracted and clarified by a buffer sufficiently emulsified with chloroform-n-butanol, then subjected to differential centrifugation and partial purification of the sucrose cushion , Sucrose gradient centrifugation, to obtain a complete granular coronavirus diameter of about 18-20nm. Purified virus preparations with a typical nuclear protein UV absorption spectra, the maximum absorption peak at about 257nm, the minimum absorption peak at about 240nm, A260 / 280 = 1.3, the highest yield of purification up to 3.4mg / kg tissue. Antiserum was prepared by immunizing rabbits with the above purified virus as antigen and the titer was 1:32 as determined by convective immunoelectrophoresis. A protein sandwich ELISA (DAS-ELISA) established by the antiserum and a double-sandwich ELISA (DAS-ELISA) of heterologous antibody used in combination with the monoclonal antibody were able to detect various banana bunchy top samples.