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[目的]观察不同深度、不同时间氦氧饱和暴露处理小鼠血清皮质酮及白介素-2(IL-2)含量的变化,探讨饱和潜水环境对免疫功能影响的规律和可能机制。[方法]将30只6～8周龄的昆明种雄性小鼠随机分为5组,分别为对照组,100m氦氧饱和暴露24h组、72h组和400m氦氧饱和暴露24h组、72h组。以酶联免疫吸附试验(ELISA)测定各组小鼠血清皮质酮及IL-2的含量。[结果]100m氦氧混合气暴露24h及72h后小鼠血清皮质酮含量均较对照组升高(P<0.05),而IL-2含量未见明显变化;400m氦氧饱和暴露24h及72h后小鼠血清皮质酮含量明显升高(P<0.01),IL-2含量明显降低(P<0.01),两种深度下暴露72h与24h组之间的皮质酮及IL-2含量差异均无统计学意义(P>0.05)。[结论]饱和潜水环境可以引起机体的应激反应,它可能是大深度饱和环境致机体免疫功能抑制的一个重要因素。
[Objective] To observe the changes of serum corticosterone and interleukin-2 (IL-2) in mice exposed to helium-oxygen saturation at different depths and different times, and to explore the regularity and possible mechanism of the effect of saturated diving environment on immune function. [Method] Thirty Kunming male mice aged 6-8 weeks were randomly divided into 5 groups: control group, 24h exposure group with 100m helioxity exposure, 72h exposure group and 400m helioxuria exposure group and 72h exposure group respectively. Serum corticosterone and IL-2 levels were measured by enzyme-linked immunosorbent assay (ELISA). [Results] The levels of serum corticosterone in serum of mice exposed to 100 m He-O were higher than those of the control group at 24 h and 72 h (P <0.05), while no significant changes were observed in the content of IL-2. After exposure to 400 m He-O and H 2 O 2 for 24 h and 72 h Serum corticosterone levels were significantly increased (P <0.01) and IL-2 levels were significantly decreased in mice (P <0.01). There were no statistic differences in corticosterone and IL-2 levels between the two groups Significance (P> 0.05). [Conclusion] Saturated submerged environment can cause the body ’s stress reaction, which may be an important factor to suppress the immune function in the deep saturation environment.