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背景:传统钙离子拮抗剂脑保护作用并不确实,盐酸法舒地尔是一种新型钙离子拮抗剂,具有强大的扩血管作用和保护缺血脑组织的作用。目的:研究盐酸法舒地尔作为细胞内钙离子拮抗剂对大鼠缺血再灌注损伤的保护作用。设计:随机对照的实验研究。地点和对象:全部实验于解放军总医院实验动物中心完成。选择体质量250～300g健康SD大鼠30只(解放军总医院实验动物中心提供),以HaruoNagasawa法建立脑缺血模型,分为盐酸法舒地尔组15只和生理盐水组15只。另选体质量250～300g健康SD大鼠50只(解放军总医院实验动物中心提供),以CarysMBannister转流法制作局灶缺血模型。转流法模型50只大鼠5只一组分为10组,各用于测定盐酸法舒地尔和生理盐水治疗动物缺血前,缺血60min,再灌注20,60,120min缺血区脑组织乳酸含量。干预:用线栓法和转流法制作大鼠大脑中动脉区缺血再灌注模型,于缺血前30min分别给予盐酸法舒地尔和生理盐水。主要观察指标:缺血后5,60min和再灌注30min的局部脑血流量(rCBF);术后3,24,48h神经功能;缺血60min,再灌注20,60,120min缺血脑组织乳酸含量。结果:法舒地尔组缺血5,60min,再灌注30min局部脑血流(rCBF)为犤(3.11±0.02),(3.60±0.02),(8.04±0.10)mL/(100g·min)犦明显高于对照组犤(2.63±0.04),(3.17±1.2
BACKGROUND: The traditional protective effect of calcium antagonists is not validated. Fasudil hydrochloride is a new type of calcium antagonist with a strong vasodilator effect and protection of ischemic brain tissue. Objective: To study the protective effect of fasudil hydrochloride as an intracellular calcium antagonist on ischemia-reperfusion injury in rats. Design: Randomized controlled experimental study. Venue and Subject: All experiments were performed at Laboratory Animal Center of Chinese PLA General Hospital. 30 healthy SD rats weighing 250-300g (provided by Experimental Animal Center of Chinese PLA General Hospital) were selected to establish cerebral ischemia model with Haruo Nagasawa method. The rats were divided into fasudil hydrochloride group and 15 saline group. Another 50 healthy 250 ~ 300g healthy SD rats (provided by the Experimental Animal Center of General Hospital of People’s Liberation Army of China) were given focal ischemia model by Carys-Bennister flow-through method. 50 rats in the commutation model were divided into 10 groups, each of which was used to measure fasudil hydrochloride and saline before ischemia, ischemia 60 min, reperfusion 20, 60, 120 min ischemia brain tissue Lactic acid content. Intervention: Rat middle cerebral artery ischemia-reperfusion model was made by thread method and flow-through method. Fasudil hydrochloride and normal saline were given 30 min before ischemia. MAIN OUTCOME MEASURES: Local cerebral blood flow (rCBF) at 5, 60 min and 30 min after reperfusion; neurological function at 3, 24 and 48 h after operation; lactic acid content in ischemic brain tissue at 60, 60, 60 min after reperfusion. Results: The local cerebral blood flow (rCBF) in fasudil group for 5,60 min and 30 min after reperfusion was (3.11 ± 0.02), (3.60 ± 0.02), (8.04 ± 0.10) mL / (100 g · min) Significantly higher than the control group 犤 (2.63 ± 0.04), (3.17 ± 1.2