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目的对分离自中国南海500m深层海水来源真菌Penicillium brocae SCSIO 05793进行菌种鉴定及次级代谢产物研究。方法通过ITS序列分析并构建系统发育树来鉴定菌株,综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、中压反相柱层析以及半制备高效液相等色谱学方法对其大米发酵产物进行分离纯化,利用核磁共振、质谱、旋光等波、光谱学手段并结合理化性质及文献数据鉴定其化学结构。结果从中分离到8个单体化合物:cyclo(L-Pro-L-Leu)(1),cyclo-(L-pro-L-Ile)(2),cyclo(L-Pro-L-Phe)(3),cyclo(L-Pro-L-Tyr)(4),3,4-二羟基苯甲酸甲酯(5),4-羟基苯甲醛(6),4-羟基苯乙酮(7),对羟基苯乙酸甲酯(8)。结论从该菌株Penicillium brocae中分离得到8个化合物。
Objective To study the fungal identification and secondary metabolites of Penicillium brocae SCSIO 05793 isolated from 500 m deep seawater in the South China Sea. Methods The ITS sequences were analyzed and phylogenetic tree was constructed to identify the strains. The strains were identified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure reverse phase column chromatography and semi-preparative HPLC method The fermentation products of rice were isolated and purified, and their chemical structures were identified by using the methods of nuclear magnetic resonance, mass spectrometry, optical rotation, spectroscopy, physicochemical properties and literature data. As a result, eight monomeric compounds were isolated from the mixture of cyclo (L-Pro-L- Leu) (1), cyclo- (L-pro-L-Ile) 3, cyclo (L-Pro-L-Tyr) (4), methyl 3,4- dihydroxybenzoate (5), 4- hydroxybenzaldehyde (6) Methyl parahydroxyphenylacetate (8). Conclusion Eight compounds were isolated from this strain Penicillium brocae.