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目的 :观察人肺癌细胞血管生成过程的病理形态学、超微结构特点及意义。方法 :采用人肺癌细胞LALU皮下移植瘤模型 ,以病理动态形态学及透射电镜观察不同时期肿瘤血管生成状态。结果 :光镜显示 ,人肺癌移植瘤第 2天至第 10天之间可分为血管生成前期和血管形成期 ,在肿瘤血管生成的第 2 0天出现肺转移灶。电镜显示 ,人肺癌移植瘤第 2天出现成血管细胞 ,第 4～ 10天 ,不成熟血管内皮细胞逐渐形成血管腔伴有较完整的新生基底板 ,内皮细胞趋向成熟发展 ,第 2 0天肿瘤血管内皮细胞更成熟 ,部分区域新生毛细血管基底板发育不全或缺陷。在以上全过程中癌细胞突起直接与成血管细胞、血管内皮细胞及血管壁相连。结论 :人肺癌细胞可诱导自身肿瘤血管生成 ,病理形态学及电镜有其特征性形态学改变 ,且与转移密切相关 ,为肺癌的血管导向治疗提供了重要依据
Objective: To observe the pathomorphology, ultrastructure and significance of angiogenesis in human lung cancer cells. Methods: Human lung cancer LALU subcutaneously transplanted tumor model was used to observe the angiogenesis status of tumor at different stages by pathological dynamic morphology and transmission electron microscopy. Results: Light microscopy showed that human lung cancer xenografts were divided into pre-angiogenesis and angiogenesis stages from day 2 to day 10, and lung metastases occurred on the 20th day of tumor angiogenesis. Electron microscopy showed that human lung cancer xenografts showed angioblasts on the second day. From day 4 to 10, immature vascular endothelial cells gradually formed blood vessels with relatively intact newborn basal lamina, endothelial cells tended to mature and developed. On the 20th day, tumors Vascular endothelial cells are more mature, some areas of newborn capillary basement hypoplasia or defects. In the whole process of cancer cells protruding directly connected with the hemangioblasts, vascular endothelial cells and vascular wall. Conclusion: Human lung cancer cells can induce their own tumor angiogenesis, pathomorphology and electron microscopy have their characteristic morphological changes, and are closely related to metastasis, providing an important basis for the vascular-oriented treatment of lung cancer