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[目的 ]探索弓形虫感染早期诊断的简便方法。 [方法 ]用SPA ELISA检测轻度、中度和重度感染弓形虫鼠尿中弓形虫循环抗原 (TCA)。 [结果 ]轻度、中度和重度感染弓形虫 3组鼠尿中均检出TCA ,上述 3组分别于弓形虫感染后d6 、d3和d4显示阳性斑点。 [结论 ]SPA ELISA能检出弓形虫感染鼠尿中的弓形虫循环抗原 ,可用于弓形虫感染的早期诊断。
[Objective] To explore a simple and convenient method for the early diagnosis of Toxoplasma gondii infection. [Method] The Toxoplasma gondii circulating antigen (TCA) of Toxoplasma gondii in mild, moderate and severe infection was detected by SPA ELISA. [Result] TCA was detected in the urine of 3 groups of Toxoplasma gondii with mild, moderate and severe infections respectively. The above three groups showed positive spots on days 6, d3 and d4 after Toxoplasma gondii infection, respectively. [Conclusion] The SPA ELISA can detect Toxoplasma gondii circulating antigen in the urine of mice infected by Toxoplasma gondii and can be used for the early diagnosis of Toxoplasma gondii infection.