有机体是由多细胞组成,机体的衰老首先表现在细胞的衰老及生理功能的下降,那么离体细胞衰老的规律就有可能在某种意义上表达了机体衰老的规律。系统研究离体细胞衰老规律的工作始于1961年Hayflick氏建立的人胚肺成纤维细胞株。由于这种细胞株保持了正常体细胞的染色体核型,因此又称二倍体细胞。进一步的研究证明,人胚肺成纤维细胞具有有限的增殖潜力(50±10次群体倍增),可以用来表达细胞水平的衰老。这个工作最终由其他研究者采用来自不同种属和不同组织的离体细胞实验观察所证实。并认为细胞的体外增殖潜力不仅与供体的组织来源有关,而且与供体种属的平均寿命和个体遗传密切相关。同时大量研究证明了供体年 Organisms are composed of multicellular cells. The aging of the body first manifests itself in the decline of cellular senescence and physiological functions. Therefore, the law of senescence in isolated cells may express the law of senescence in a certain sense. The systematic study of the laws of senescence in vitro began with the human embryonic lung fibroblast cell line established by Hayflick in 1961. As this cell line to maintain the normal somatic chromosome karyotype, it is also called diploid cells. Further studies demonstrated that human embryonic lung fibroblasts have limited proliferative potential (50 ± 10 population doublings) and can be used to express cell-level senescence. This work was eventually confirmed by other investigators using in vitro cell-based observations from different species and tissues. And that the potential of cell proliferation in vitro is not only related to the donor tissue source, but also with the average donor life expectancy and individual genetic closely related. At the same time, a large number of studies have proved the donor years