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利用RAPD标记研究选自杉木第2代育种试验林、拟纳入高世代育种的182个优良基因型的遗传变异。29条随机引物扩增出311条谱带,其中多态性谱带245条(78.8%)。聚类分析表明,182个优良基因型可聚为11类。AMOVA分析表明,96.8%的遗传变异存在于第2代育种试验林内,而试验林间的遗传变异虽然显著(Φst=0.032,P<0.001),但比例较低(3.2%)。研究结果表明,杉木第2代育种群体存在高水平的遗传多样性,显示了进一步育种的良好潜力。
The RAPD marker was used to study the genetic variation of 182 elite genotypes selected from the second-generation Chinese fir plantation to be included in the breeding of high-generational generations. There were 311 bands amplified by 29 random primers, of which 245 bands (78.8%) were polymorphic bands. Cluster analysis showed that 182 excellent genotypes could be clustered into 11 categories. The AMOVA analysis showed that 96.8% of the genetic variation existed in the 2nd generation breeding trial, while the genetic variation among the experimental stands was significant (Φst = 0.032, P <0.001) but lower (3.2%). The results showed that there was a high level of genetic diversity in the second generation breeding population of Chinese fir and showed the good potential of further breeding.