论文部分内容阅读
目的:探讨Temsirolimus对腺样囊性癌ACC-M细胞株自噬水平的影响,以研究该药物诱导的自噬对腺样囊性癌细胞的作用。方法:通过细胞增殖实验研究Temsirolimus对ACC-M细胞增殖的影响;通过Western印迹检测实验组与对照组微管相关蛋白1轻链3(LC3)和Beclin1的表达差异;利用透射电镜观察ACC-M细胞中自噬体的形态及数量,采用SPSS15.0软件包对实验结果进行t检验。结果:Temsirolimus对ACC-M细胞具有明显的生长抑制作用,并呈现出剂量-效应关系;LC3和Beclin1在实验组细胞中表达水平高于对照组(P<0.05);透射电镜实验中,实验组细胞胞内自噬体及自噬溶酶体数量明显高于对照组(P<0.01)。结论:Temsirolimus通过诱导ACC-M细胞自噬,产生了明显的抑制肿瘤细胞生长的作用,提示细胞自噬是Temsirolimus作用于唾液腺腺样囊性癌的重要抗肿瘤机制。
OBJECTIVE: To investigate the effect of Temsirolimus on autophagy in ACC-M cell line of adenoid cystic carcinoma in order to study its effect on adenoid cystic carcinoma cells. METHODS: The effects of Temsirolimus on the proliferation of ACC-M cells were studied by cell proliferation assay. The expression of microtubule-associated protein 1, light chain 3 (LC3) and Beclin1 in the experimental and control groups were detected by Western blotting. The morphology and number of autophagosomes in the cells were tested by SPSS 15.0 package on the experimental results. RESULTS: Temsirolimus had a significant growth-inhibitory effect on ACC-M cells with a dose-response relationship. The expression levels of LC3 and Beclin1 in the experimental group were higher than those in the control group (P <0.05). In the experimental group The number of intracellular autophagosomes and autophagy lysosomes was significantly higher than that of the control group (P <0.01). CONCLUSIONS: Temsirolimus can induce autophagy in ACC-M cells and significantly inhibit the growth of tumor cells. It suggests that autophagy is an important anti-tumor mechanism of Temsirolimus in salivary adenoid cystic carcinoma.