采用RT-PCR及RACE的方法从东方山羊豆中克隆了一种新的Na+/H+逆向转运蛋白基因。该基因全长2280bp,开放阅读框为1626bp,编码542个氨基酸残基。预测该蛋白分子量为60.0kDa,可能有10个跨膜区域。RT-PCR检测结果表明,在NaCl胁迫条件下东方山羊豆GoNHX1基因可在根、茎、叶中诱导表达,在叶中的表达量随着处理时间的延长而逐渐增加,到24h表达量下降。 A new Na + / H + antiporter gene was cloned from oriental goat by RT-PCR and RACE. The gene is 2280bp in length and 1626bp in open reading frame, encoding 542 amino acid residues. The molecular weight of this protein is predicted to be 60.0 kDa, and there may be 10 transmembrane regions. The results of RT-PCR indicated that GoNHX1 gene was induced in roots, stems and leaves of Arabidopsis thaliana under NaCl stress and its expression gradually increased with the prolongation of treatment time.