论文部分内容阅读
在磷酸盐缓冲溶液(PBS)中,以邻苯二胺(o-PD)和没食子酸(GA)为功能单体,水胺硫磷(ICP)为模板分子,采用电位循环扫描法在玻碳电极(GCE)表面进行电聚合,形成绝缘性的分子印迹膜,通过电化学阻抗谱(EIS)和循环伏安法(CV)对分子印迹膜进行表征。在最佳实验条件下,以铁氰化钾[K3Fe(CN)6]为探针,用差分脉冲伏安法(DPV)对ICP进行定量分析。该传感器在2.00×10-7～2.00×10-4mol/L范围内,传感器的响应信号与ICP的浓度呈良好的线性关系,检测限为6.06×10-8mol/L(S/N=3)。将其用于蔬果等实际样品中ICP的测定,回收率为95.6%～104.0%。
In phosphate buffered saline (PBS), ortho-phenylenediamine (o-PD) and gallic acid (GA) were used as functional monomers and aqueous ammonia was used as template molecule. The surface of GCE was electropolymerized to form an insulating molecular imprinted membrane. The molecular imprinted membrane was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Under the optimal experimental conditions, ICP was quantitatively analyzed by differential pulse voltammetry (DPV) using potassium ferricyanide [K3Fe (CN) 6] as a probe. The sensor has a good linear relationship between the response of the sensor and the concentration of ICP in the range of 2.00 × 10-7 ~ 2.00 × 10-4mol / L with the detection limit of 6.06 × 10-8mol / L (S / N = 3) . The method was applied to the determination of ICP in real samples such as fruits and vegetables. The recoveries ranged from 95.6% to 104.0%.