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目的 :观察哮喘小鼠T细胞周期和bcl- 2基因表达的变化及地塞米松对它们的影响。方法 :复制小鼠过敏性哮喘动物模型 ,应用流式细胞术观察脾及肺泡灌洗液 (BALF)中T细胞数、T细胞周期和bcl- 2基因表达的变化。结果 :哮喘组脾和BALF中淋巴细胞CD3表达率显著高于对照组 ;哮喘 +地塞米松组BALF淋巴细胞中CD3表达降低 ,但脾淋巴细胞中CD3表达增高 ;哮喘组S期及G2 +M期细胞数明显多于对照组 ,同时凋亡率也高于对照组 ;哮喘 +地塞米松组S期及G2 +M期细胞数减少 ,凋亡增多 ;哮喘组T细胞bcl- 2基因表达率显著高于对照组 ,哮喘 +地塞米松对bcl- 2基因表达与哮喘组比较无明显变化。结论 :哮喘发病时 ,T细胞增多 ,脾T细胞活化增殖增加 ,凋亡增加 ,同时 ,bcl- 2基因表达率明显增加。地塞米松对哮喘的治疗作用可能并非通过抑制T细胞bcl- 2基因表达的途径。
Objective: To observe the changes of T cell cycle and bcl-2 gene expression in asthmatic mice and the effect of dexamethasone on them. Methods: Animal models of allergic asthma in mice were established. The changes of T cell number, T cell cycle and bcl-2 gene expression in the spleen and BALF were observed by flow cytometry. Results: The expression of CD3 in BALF and lymphocytes in asthma group was significantly higher than that in control group. CD3 expression in BALF lymphocytes in asthma + dexamethasone group was decreased, but CD3 expression in splenic lymphocytes was increased. In S phase and G2 + M The number of serous cells in asthma group was significantly more than that in control group, and the apoptosis rate was also higher than that in control group. The number of cells in S phase and G2 + M phase in asthma + dexamethasone group was decreased and the apoptosis rate was increased. The expression of bcl- Significantly higher than the control group, asthma + dexamethasone on bcl-2 gene expression compared with the asthma group no significant change. Conclusion: The incidence of asthma, T cells increased, splenic T cell activation and proliferation increased, apoptosis increased, while bcl-2 gene expression was significantly increased. The therapeutic effect of dexamethasone on asthma may not be through inhibiting the expression of bcl-2 gene in T cells.