本实验用大鼠灌服醋酸棉酚(每日30mg/kg体重)7、8、9和10周后,应用淘析器细胞分离术,获得了高纯度的粗线期精母细胞作~3H-精氨酸掺入能力的液闪测定,计算单位组蛋白重量中放射活性的脉冲数。结果表明,与对照组相比,棉酚组的粗线期精母细胞摄取精氨酸能力降低了35～50%。证明棉酚不仅对晚期精子细胞有影响,也使初级精母细胞的组蛋白合成代谢受到明显干扰。本文还讨论了有关的作用机理。 In this experiment, rats gavage acetic acid gossypol (daily 30mg / kg body weight) 7,8,9 and 10 weeks after the application of elutriation cell isolation, obtained high-purity pachytene spermatocytes for ~ 3H - Determination of arginine incorporation ability by liquid flasks to calculate the number of radioactive pulses per unit of histone weight. The results showed that compared with the control group, the ginsenoside group reduced pomegranate arginine capacity by 35-50%. Gossypol proved not only to affect the late sperm cells, but also to primary spermatocyte histone synthesis was significantly disturbed. This article also discusses the mechanism of action.