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目的:利用不同剂量X射线对体外培养肝癌细胞HepG2进行照射,研究人宫颈癌基因(HCCR)及其相关基因对于细胞增殖的影响及其可能的分子机制。方法:采用0、1.0、2.0、4.0 Gy的吸收剂量X射线照射细胞,用克隆形成法观察细胞的存活情况;光镜下观察细胞形态变化;同时在辐照24 h后用RT-PCR法检测细胞中HCCR、p53、Bax mRNA的表达情况。结果:1.0、2.0、4.0 Gy吸收剂量时克隆形成率分别为(76.13±3.52)%、(62.22±2.17)%、(25.35±3.10)%,随着辐射剂量的增加细胞存活率显著下降(P<0.05)。HCCR mRNA相对表达量分别为0.95±0.04、0.8±0.07、0.45±0.03;p53 mRNA相对表达量分别为1.07±0.22、2.02±0.34、2.90±0.52;Bax mRNA相对表达量分别为1.12±0.11、1.63±0.36、2.48±0.27。较空白对照组,2Gy、4Gy剂量照射组HCCR mRNA相对表达量显著降低(P<0.05),而p53、Bax mRNA相对表达量显著增加(P<0.05)。结论:X射线照射可下调肝癌细胞HepG2的HCCR-1表达,抑制细胞增殖,作用机制可能与其相关基因p53、Bax表达升高有关。
OBJECTIVE: To study the effect of human cervical cancer gene (HCCR) and its related genes on cell proliferation and its possible molecular mechanism by using different doses of X-rays to irradiate HepG2 hepatoma cells in vitro. Methods: The cells were irradiated with 0, 1.0, 2.0 and 4.0 Gy X-ray. The cell viability was observed by colony formation assay. The morphological changes of cells were observed under light microscope. At 24 hours after irradiation, HCCR, p53, Bax mRNA expression in the cells. RESULTS: The cell formation rates at the doses of 1.0, 2.0 and 4.0 Gy were (76.13 ± 3.52)%, (62.22 ± 2.17)% and (25.35 ± 3.10)%, respectively. <0.05). The relative expression of HCCR mRNA was 0.95 ± 0.04, 0.8 ± 0.07 and 0.45 ± 0.03, respectively. The relative expression of p53 mRNA was 1.07 ± 0.22, 2. 02 ± 0.34 and 2.90 ± 0.52, respectively. The relative expression of Bax mRNA was 1.12 ± 0.11 and 1.63 respectively ± 0.36, 2.48 ± 0.27. Compared with the blank control group, the relative expression of HCCR mRNA in 2Gy and 4Gy groups was significantly decreased (P <0.05), while the relative expression of p53 and Bax mRNA was significantly increased (P <0.05). Conclusion: X-ray irradiation can down-regulate the expression of HCCR-1 in HepG2 cells and inhibit the proliferation of HepG2 cells. The mechanism may be related to the increased expression of p53 and Bax.