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目的研究牙龈炎症对环孢菌素A(CsA)致牙龈增生的影响及转化生长因子-β(1TGF-β1)在其中的作用。方法 2006年5—8月于南京军区福州总医院比较医学科,将34只雄性SD大鼠随机分为4组,其中对照组7只,胃饲橄榄油9周后处死;结扎组7只,右侧下颌第一磨牙牙颈部栓结正畸用细钢丝,胃饲橄榄油9周后处死;单纯用药组10只,胃饲CsA橄榄油溶液(每天30mg/kg)9周后处死;结扎+用药组10只,右侧下颌第一磨牙牙颈部栓结正畸用细钢丝,胃饲CsA橄榄油溶液9周处死。取下颌第一磨牙做病理切片,苏木素-伊红(HE)染色观察牙周组织变化;酶联免疫吸附试验(ELISA)检测大鼠血清中TGF-β1表达变化。结果胃饲橄榄油或CSA橄榄油溶液9周,结扎+用药组大鼠牙龈增生最明显,其舌侧牙龈结缔组织面积为(294705.30±2490.13)μm2,明显高于单纯用药组[(48710.54±3695.88)μm2]、结扎组[(21440.15±2735.85)μm2]和对照组[(17911.53±1672.70)μm2](P<0.05);结扎+用药组和单纯用药组大鼠血清中TGF-β1质量浓度分别为(276.60±99.79)ng/mL和(320.11±68.27)ng/mL,均明显高于结扎组[(77.80±54.57)ng/mL]和对照组[(67.63±41.55)ng/mL](P<0.05)。结论在炎症存在下,CsA诱发的牙龈增生会更明显;TGF-β1在CsA诱发的牙龈增生中起一定的作用。
Objective To investigate the effect of gingival inflammation on gingival hyperplasia induced by cyclosporin A (CsA) and the role of transforming growth factor-β (TGF-β1) in gingivitis. METHODS: From May to August 2006, Fuzhou General Hospital of Nanjing Military Region, 34 male SD rats were randomly divided into 4 groups, including 7 in the control group and 9 weeks after gastric feeding of olive oil. Seven rats in the ligation group were sacrificed, The right molar mandibular first molars were tied with orthodontic wire, and the rats were sacrificed after 9 weeks of gastric feeding with olive oil. 10 rats in the treatment group were given CsA olive oil solution (30mg / kg daily) for 9 weeks and then sacrificed. + Drug group 10, the right molar mandibular first molar teeth tied with orthodontic wire, gastric feeding CsA olive oil solution was sacrificed 9 weeks. The mandibular first molar was removed for histopathological examination. The changes of periodontal tissues were observed by hematoxylin and eosin (HE) staining. The changes of TGF-β1 expression in serum were detected by enzyme linked immunosorbent assay (ELISA). Results After gavage with olive oil or CSA olive oil for 9 weeks, the gingival hyperplasia was the most obvious in the ligation + treatment group. The gingival connective tissue area on the lingual side was (294705.30 ± 2490.13) μm2, which was significantly higher than that in the pure drug group [(48710.54 ± 3695.88 ) in the ligation group [(21440.15 ± 2735.85) μm2] and the control group [(17911.53 ± 1672.70) μm2] (P <0.05). The concentrations of TGF-β1 in the ligation group and the pure drug group were (276.60 ± 99.79) ng / mL and (320.11 ± 68.27) ng / mL were significantly higher than those in the ligation group [(77.80 ± 54.57) ng / mL and the control group 0.05). Conclusion CsA-induced gingival hyperplasia is more obvious in the presence of inflammation. TGF-β1 plays a role in CsA-induced gingival hyperplasia.