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Objective To detect the patients with IgA nephropathy by glycobilolgy and immunological technique.Methods An helia aspera lectin-ELISA immunoassay was developed to discriminate the aberrantly glycosylated IgA in serum from normal IgA molecules by using HAA lectin, which reacts preferentially with the GalNAc of the terminal sugar chain, and AKP conjugated specific IgA antibody.Results The results given by A405 nm values from 156 controls, 22 patients with IgAN and 19 patients with uncertain IgAN were 0.22±0.10, 0.68±0.17 and 0.38±0.15, respectively. The significance between the controls and the patients with IgAN was observed (P<0.001).Conclusion This assay is sensitive, specific and valuable for the diagnosis of patients with IgA nephropathy.