In the present paper we introduce and study pre-T0-,pre-R0-,pre-R1-,pre-T2(pre-Hausdorff)-,pre-T3(pre-regularity)-,pre-T4(pre-normality)-,pre-strong T3- and pre-strong T4-separation axioms in fuzzifying topology and give some of their characterisations as well as the relations of these axioms and other separation axioms in fuzzifying topology introduced by Shen[7].