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用由溶组织内阿米巴致病株（ＨＭ－１：ＩＭＳＳ）为免疫原诱导产生的特异性单克隆抗体（ＭｃＡｂ）４Ｇ６，与从急性阿米巴痢疾患者或包囊携带者分离的溶组织内阿米巴（Ｅｈ）滋养体进行间接荧光抗体反应和淀粉凝胶电泳同工酶分析。显示，从有发热、腹泻、脓血便患者体内分离的虫株与ＭｃＡｂ４Ｇ６出现阳性反应，滴度达１：５１２０；同工酶分析也属于致病性酶株群Ⅱ。而有发热、腹泻、无脓血便患者体内分离的虫体与ＭｃＡｂ４Ｇ６均为阴性反应，且为非致病性酶株群Ⅰ。提示，致病性虫株与非致病性虫株的免疫原性不同，应用本法可准确鉴别致病性虫株，且简便、价廉、快速，还可用于流行病学调查。
The specific monoclonal antibody (McAb) 4G6 induced by the immunogenicity of Entamoeba histolytica (HM-1: IMSS) was used in combination with a monoclonal antibody isolated from patients with acute amebiasis or encapsulated carriers Tissue Eh trophozoites were subjected to indirect fluorescent antibody reaction and starch gel electrophoresis isozyme analysis. Showed that there was a positive reaction with McAb4G6 in the isolates from patients with fever, diarrhea and sepsis, with titer of 1: 5120; isozyme analysis also belonged to pathogenic enzyme group Ⅱ. In contrast, McAb4G6, a member of fever, diarrhea and abscesses, was negatively associated with the non-pathogenic enzyme group Ⅰ. It is suggested that the immunogenicity of the pathogenic and non-pathogenic strains is different. This method can accurately identify the pathogenic insect strains and is simple, inexpensive, rapid and can be used for epidemiological investigation.