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目的:探讨长链非编码RNA H19在前列腺癌组织中的表达及其对前列腺癌细胞DU145和PC-3糖代谢和生长的影响。方法:采用实时荧光定量PCR(q PCR)检测20例前列腺癌组织(高分化和低分化各10例)和5例良性前例腺增生组织中H19的表达水平。H19小干扰RNA(siRNA)转染前例腺癌细胞DU145和PC-3,以不转染载体和转染阴性载体作为空白对照组和阴性对照组,MTT法检测转染后DU145和PC-3细胞的生长情况,q PCR检测转染细胞中H19的表达水平,通过测定培养液中葡萄糖和乳酸的含量分析前例腺癌细胞糖代谢的变化。结果:高分化和低分化前例腺癌组织中H19的相对表达量(0.725±0.385、2.086±0.542)均显著高于BPH组织(0.210±0.068),P均<0.01,且低分化前例腺癌组织显著高于高分化前列腺癌组织(P<0.01)。转染H19 siRNA后,H19在DU145和PC-3细胞中的表达均显著低于空白对照组和阴性对照组(P均<0.01);转染H19 siRNA后的DU145和PC-3细胞,生长能力、葡萄糖消耗量和乳酸生成量均较空白对照组和阴性对照组显著下降(P均<0.01)。结论:H19在前列腺癌中呈现高表达,抑制其表达可有效抑制前列腺癌细胞的糖代谢和生长。
OBJECTIVE: To investigate the expression of long-chain non-coding RNA H19 in prostate cancer and its effect on the glucose metabolism and growth of prostate cancer cells DU145 and PC-3. Methods: The expression of H19 in 20 cases of prostate cancer tissues (10 cases of well-differentiated and poorly differentiated) and 5 cases of benign prostatic hyperplasia tissues were detected by real-time fluorescence quantitative PCR (qPCR). H19 small interfering RNA (siRNA) was transfected into DU145 and PC-3 cells. The transfected DU145 and PC-3 cells were transfected with the untransfected and transfected negative vectors as blank control and negative control. The expression of H19 in transfected cells was detected by q PCR. The changes of glucose metabolism in the former adenocarcinoma cells were analyzed by measuring the contents of glucose and lactate in the culture medium. Results: The relative expression of H19 in well-differentiated and poor-differentiated adenocarcinoma tissues was significantly higher than that in BPH tissues (0.725 ± 0.385 and 2.086 ± 0.542, P <0.01) Significantly higher than that of well-differentiated prostate cancer (P <0.01). After transfected with H19 siRNA, the expression of H19 in DU145 and PC-3 cells was significantly lower than that in the blank control group and the negative control group (all P <0.01). The growth of DU145 and PC-3 cells transfected with H19 siRNA , Glucose consumption and lactate production were significantly decreased compared with the blank control group and the negative control group (all P <0.01). Conclusion: H19 is highly expressed in prostate cancer. Suppression of its expression can effectively inhibit the glucose metabolism and growth of prostate cancer cells.