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目的:建立采用高效液相色谱法测定泻痢停片中磺胺甲噁唑含量的方法。方法:色谱柱为Diamonsil~(TM)C_(18)(5μm,200mm×4.6mm),流动相为乙腈-水-磷酸(50:50:0.1),每1000 ml流动相中加十二烷基硫酸钠3.5g,流速1 ml·min~(-1),检测波长为257 nm。结果:磺胺甲噁唑平均回收率为98.7％。结论:方法简便,结果准确可靠。
Objective: To establish a method for the determination of sulfamethoxazole in Xiesan tablets by high performance liquid chromatography. METHODS: Diamonsil TM C 18 (5μm, 200mm × 4.6mm) was used as the mobile phase. The mobile phase was acetonitrile-water-phosphoric acid (50:50: 0.1) 3.5g of sodium sulfate, the flow rate of 1 ml · min ~ (-1), the detection wavelength of 257 nm. Results: The average recovery of sulfamethoxazole was 98.7%. Conclusion: The method is simple and the result is accurate and reliable.